Complexation and immobilization of arsenic in maize using green synthesized silicon nanoparticles (SiNPs).

Arsenic (As) is a heavy metal that is toxic to both plants and animals. Silicon nanoparticles (SiNPs) can alleviate the detrimental effects of heavy metals on plants, but the underlying mechanisms remain unclear. The study aims to synthesize SiNPs and reveal how they promote plant health in Arsenic-polluted soil. 0 and 100% v/v SiNPs were applied to soil, and Arsenic 0 and 3.2 g/ml were applied twice. Maize growth was monitored until maturity. Small, irregular, spherical, smooth, and non-agglomerated SiNPs with a peak absorbance of 400 nm were synthesized from Pycreus polystachyos. The SiNPs (100%) assisted in the development of a deep, prolific root structure that aided hydraulic conductance and gave mechanical support to the maize plant under As stress. Thus, there was a 40-50% increase in growth, tripled yield weights, and accelerated flowering, fruiting, and senescence. SiNPs caused immobilization (As(III)=SiNPs) of As in the soil and induced root exudates Phytochelatins (PCs) (desGly-PC2 and Oxidized Glutathione) which may lead to formation of SiNPs=As(III)-PCs complexes and sequestration of As in the plant biomass. Moreover, SiNPs may alleviate Arsenic stress by serving as co-enzymes that activate the antioxidant-defensive mechanisms of the shoot and root. Thus, above 70%, most reactive ROS (OH) were scavenged, which was evident in the reduced MDA content that strengthened the plasma membrane to support selective ion absorption of SiNPs in place of Arsenic. We conclude that SiNPs can alleviate As stress through sequestration with PCs, improve root hydraulic conductance, antioxidant activity, and membrane stability in maize plants, and could be a potential tool to promote heavy metal stress resilience in the field.

Impacts of anthropogenic disturbance on forest vegetation cover, health, and diversity within Doma forest reserve, Nigeria.

Forest encroachment is a common practice that has led to the destruction of canopy trees in the Guinea savanna part of Nigeria. This study investigated the influence of human activities on vegetation health and species composition of Doma forest reserve located in Nasarawa State, Nigeria. Landsat satellite data from 1986 to 2021 were utilized to assess forest cover change, land surface temperature (LST), and vegetation indices (VIs). The results show that dense woodland vegetation in the Doma forest reserve depreciated between 1991 and 1999 by 17.82% before increasing by 7.37% between 1999 and 2021. Similarly, vegetation greenness (measured by the Normalized Difference Vegetation Index (NDVI), Green Chlorophyll Vegetation Index (GCVI), and leaf area index (LAI)) of the forest mirrored the changes observed in the forest cover. The LST extracted for each year was correlated with all VIs, and an inverse relationship was observed in all relationships analyzed. The decline in greenness between 1999 and 2011 was attributed to increasing lumbering, bush burning, and sand dredging activities. Results also showed the current diversity state (H1 = 0.23), evenness (0.63), and the volume of tree (1.31 m3) species in the heart of the Doma forest reserve. However, a high (25%) native tree species in the Fabaceae family correlated with a dramatic increase in the VIs and an increase in dense woodland cover indicating the importance of Fabaceae in forest ecosystem regeneration.

Anatomical adaptation of water-stressed Eugenia uniflora using green synthesized silver nanoparticles and melatonin.

Cell and sub-cellular anatomical adjustments are adaptations utilized by plants to tolerate abiotic stress. Both melatonin and Morinda lucida-silver nanoparticles (ML-AgNPs) are recognized as bio-stimulants. The study examined the morphological changes and adaptive characteristics of these bio-stimulants under water-stress Eugenia uniflora. Twenty-four hours was spent priming the seeds with melatonin (0.06 mg/L), ML-AgNPs (0.06 mg/L), and a mixture (1:1) of the two. The seeds were sown and subjected to water stress for 7 days. The leaves, stems, and roots of water-stressed E. uniflora were sectioned, dried, and examined using a microscope. Drought stress led to the production of non-glandular trichomes on the abaxial and the transformation of paracytic stomata into diacytic stomata. During water stress, melatonin enlarges intercellular gaps and stomata, increases sponge and palisade parenchyma, and thickens epidermis (stem and root) and fibers. The ML-AgNPs diminished the size of mesophyll, intercellular gaps, stomata, and stem fiber. The ML-AgNPs increased the size of bulliform cells and activated the mechanical resistance features of sclerophyllous leaves (thick-celled epidermis and sclerieds) and ray parenchyma (root and stem). Equally, Melatonin and ML-AgNPs increased stem and root anatomical characteristics (xylem, bark, pith, cortex, epidermis, and vascular bundles). Stomata of E. uniflora are susceptible to alterations and undergo cell division into two new stomata (stomatogensis) in response to varying conditions (melatonin and ML-AgNPs). Melatonin adopted a strategy for maintaining a high plant water status, possibly by osmoregulation, whereas E. uniflora primed with ML-AgNPs survived by minimizing transpirational water loss through morphological changes.

Green synthesis and characterization of silver nanoparticles using Morinda lucida leaf extract and evaluation of its antioxidant and antimicrobial activity.

This study emphasizes the production of eco-friendly silver nanoparticles from a medicinal plant extract of Morinda lucida (M. lucida) and investigated its antioxidant and antimicrobial activity. Phytochemical screening of M. lucida (ML) leave extract was carried out and observed to contain some fundamental phyto-reducing agents such as reducing sugar, proteins, and alkaloids. The green synthesized AgNPs (ML-AgNPs) were characterized by UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), transmission emission microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), and Energy dispersive X-ray analysis (EDX). Thermo gravimetric analysis (TGA) was performed on the synthesized ML-capped AgNPs to determine the thermal stability and the formation of the green synthesized AgNPs. The formation of AgNPs was confirmed by the UV-vis absorption spectra, which showed an absorption band at 420 nm. The morphology of ML extract-mediated AgNPs was mostly spherical and rough-edged crystallite nanostructures, with an average particle size of 11 nm. The FTIR analyses revealed distinctive functional groups which were directly involved in the synthesis and stability of AgNPs. The crystallite size was 8.79 nm, with four intense peaks at 2θ angles of 38°, 44°, 64°, and 77°. At an energy level of 3.4 keV, a significant signal was observed indicating the production of thermally stable and pure crystallite AgNPs. The antioxidant property of green synthesized ML-AgNPs was determined to be 40% higher than that of crude M. lucida leaf extract. The ability of green synthesized ML-AgNPs to scavenge free radicals also increased in the order of OH- < NO < H2O2. The ML-AgNPs have strong activities with a maximum against P. vulgaris and a minimum with E. faecalis.

The impact of tantalite mining on the flora diversity in Nasarawa state, Nigeria.

The activities of the artisanals and small-scale miners in Nasarawa state, Nigeria, are increasing daily without considering the loss of biodiversity, which has continuously disrupted ecological functions and environmental balance. The study aimed at investigating the effect of tantalite mining activities on flora diversity. Three study sites were selected for floristic data collection, comprising tantalite mining site A situated in Azara, Awe LGA; tantalite mining site B situated in Tunga, Awe LGA; and referred site C situated along the Makurdi-Obi Road, Lafia LGA. Eight plots of 20 × 20 m were systematically placed along two transect lines of 1000 m with 500 m distance apart. The total numbers of 32 plant species, at the ratio of 11:20:21 individual species, were found in sites A, B, and referred site C, respectively. Tantalite mining site A showed 46% rare herbs dominating the site, with an introduction of new non-native species of Jateorhiza spp. and Hyptis suaveolens, presenting a significantly high number of individuals (p ˂ 0.0069). Tantalite site B, Tunga, had 50% reductions of several indigenous tree species such as Daniellia oliveri and Vitex doniana, while site A had 75% reduction of tree species. Therefore, the floristic diversity in site A endured a higher degradation than in site B. The indigenous species in site A were almost completely replaced with problematic weeds, invasive weed species, and non-native plant species. The tree diversities in sites A and B were seriously threatened, and H. suaveolens has been regarded as a potentially invasive plant species in Nasarawa state, Nigeria.